PUBLICATIONS (2015-2023)

PUBLICATIONS(2015-2023)

Pérez Mora, W.H., Castillejo, M.A., Jorrín Novo, J.V., Melgarejo, L.M., Ardila, H.D. Thiamine-induced resistance in carnation against Fusarium oxysporum f. sp dianthi and mode of action studies based on the proteomics analysis of root tissue. Scientia Horticulturae. Aceptado.

Rodríguez de Francisco, L. E., Carreras-De León, R., Navarro Cerrillo, R. M., Paulino-Gervacio, L. A., Rey, M. D., del Orbe Matos, D., … & Jorrín Novo, J. V. (2022). Population genetic structure and dispersal of Pinus occidentalis in the Dominican Republic by chloroplastic SSR, with implications for its conservation, management, and reforestation. Canadian Journal of Forest Research, 52(4), 553-560.

Salas-Moreno, M., Castillejo, M. A., Lopez-Hidalgo, C., Marrugo-Negrete, J., Rodriguez-Cavallo, E., Mendez-Cuadro, D., & Jorrin-Novo, J. (2022). LC-MS/MS shotgun proteomics reveals biochemical mechanisms of Paspalum fasciculatum tolerance to Pb-stress. BIOLOGIA PLANTARUM, 66, 188-200.

Salas-Moreno, M., Castillejo, M. A., Rodríguez-Cavallo, E., Marrugo-Negrete, J., Mendez-Cuadro, D., & Jorrin-Novo, J. (2022). Proteomic changes in Paspalum fasciculatum leaves exposed to Cd stress. Plants, 11: 2455.

Guerrero-Sánchez, V. M., López-Hidalgo, C., Rey, M. D., Castillejo, M. Á., Jorrín-Novo, J. V., & Escandón, M. (2022). Multiomic Data Integration in the Analysis of Drought-Responsive Mechanisms in Quercus ilex Seedlings. Plants, 11: 3067.

Ogawa, M., Carmona-Jiménez, P., García-Martínez, T., Jorrín-Novo, J. V., Moreno, J., Rey, M. D., & Moreno-García, J. (2022). Use of yeast biocapsules as a fungal-based immobilized cell technology for Indian Pale Ale-type beer brewing. Applied Microbiology and Biotechnology, 1-11.

González-Guzmán, A., Rey, M. D., Froussart, E., & Quesada-Moraga, E. (2022). Elucidating the effect of endophytic entomopathogenic fungi on bread wheat growth through signaling of immune response-related hormones. Applied and Environmental Microbiology, 88(18), e00882-22.

Escandón, M., Bigatton, E. D., Guerrero-Sánchez, V. M., Hernández-Lao, T., Rey, M. D., Jorrín-Novo, J. V., & Castillejo, M. A. (2022). Identification of proteases and protease inhibitors in seeds of the recalcitrant forest tree species Quercus ilex. Frontiers in Plant Science, 13.

Tienda-Parrilla, M., López-Hidalgo, C., Guerrero-Sanchez, V. M., Infantes-González, Á., Valderrama-Fernández, R., Castillejo, M. Á., … & Rey, M. D. (2022). Untargeted MS-based metabolomics analysis of the responses to drought stress in Quercus ilex L. leaf seedlings and the identification of putative compounds related to tolerance. Forests, 13(4), 551.

San-Eufrasio, B., Castillejo, M. Á., Labella-Ortega, M., Ruiz-Gómez, F. J., Navarro-Cerrillo, R. M., Tienda-Parrilla, M., … & Rey, M. D. (2021). Effect and response of Quercus ilex subsp. ballota [Desf.] Samp. seedlings from three contrasting Andalusian populations to individual and combined Phytophthora cinnamomi and drought stresses. Frontiers in Plant Science, 12, 722802.

Amaral, J., Lamelas, L., Valledor, L., Castillejo, M.A., Alves, A., Pinto, G. 2021. Comparative proteomics of Pinus-Fusarium circinatum interactions reveal metabolic clues to biotic stress resistance. Physiologia Plantarum, 173:2142-2154.

Chalbi, A., Sghaier-Hammami, B., Baazaoui, N., Hammami, S. B., Hatem, B. J., Garcia-Caparros, P., … & Abdelly, C. (2021). Comparative study of the effect of salt stress, Alternaria alternata attack or combined stress on the Cakile maritima growth and physiological performance. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(3), 12446-12446.

Castillejo, M.A., Villegas-Fernández, A., Hernandez-Lao, T., Rubiales, D. 2021. Photosystem II repair cycle in faba bean may play a role in its resistance to Botrytis fabae infection. Agronomy, 11, 2247.

Morales, C. J., de Francisco, L. E. R., Hidalgo, C. L., Cerrillo, R. M. N., Perdomo, O. P., & Novo, J. V. J. (2021). Endemic Juniperus gracilior varieties of the Hispaniola island, tree taxa of environmental and economic relevance and a valuable phytochemical source. Bosque, 42, 7-22.

Tartaglia, M., Sciarrillo, R., Zuzolo, D., Amoresano, A., Illiano, A., Pinto, G., … & Guarino, C. (2021). Why consumers prefer green friariello pepper: changes in the protein and metabolite profiles along the ripening. Frontiers in Plant Science, 12, 668562.

Valero-Galván J, González-Fernández R, Jorrín-Novo JV. 2021. Interspecific variation between the American Quercus virginiana and Mediterranean Quercus species in terms of seed nutritional composition, phytochemical content, and antioxidant activity. Molecules 26:2351.

Sánchez‑Rodríguez AR, Rey MD, Nechate‑Drif H, Castillejo-Sánchez MA, Jorrín‑Novo JV, Torrent J, del Campillo MC, Sacristán D. 2021 Combining P and Zn fertilization to enhance yield and grain quality in maize grown on Mediterranean soils. Scientific Reports 11:7427

San-Eufrasio B, Bigatton ED, Guerrero-Sánchez VM, Chaturvedi P, Jorrín-Novo JV, Rey MD, Castillejo-Sánchez MA. 2021. Proteomics data analysis for the identification of proteins and derived proteotypic peptides of potential use as putative drought tolerance markers for Quercus ilex. International Journal of Molecular Sciences 22:3191

Escandón M, Jorrín-Novo JV, Castillejo-Sánchez MA. 2021. Application and optimization of label-free shotgun approaches in the study of Quercus ilex. Journal of Proteomics 233:104082.

Sghaier-Hammami B, Castillejo-Sánchez MA, Baazaoui N, Jorrín-Novo JV, Escandón M. 2021. GeLC-Orbitrap/MS and 2-DE-MALDI-TOF/TOF comparative proteomics analysis of seed cotyledons from the non-orthodox Quercus ilex tree species. Journal of Proteomics 233:104087

Kulak M, Jorrín-Novo JV, Romero-Rodríguez MC, Yildirim ED, Gul F, Karaman S. 2021. Seed priming with salicylic acid on plant growth and essential oil composition in basil (Ocimum basilicum L.) plants grown under water stress conditions. Industrial Crop & Products 161:113235

Rey MD, Ramírez C, Martín AC. 2021. Wheat, Rye, and Barley Genomes Can Associate during Meiosis in Newly Synthesized Trigeneric Hybrids. Plants 10:113.

López-Hidalgo C, Trigueros Peña M, Menéndez M, Jorrín-Novo JV. 2020. Phytochemical composition and variability in Quercus ilex acorn morphotypes as determined by NIRS and MS-based approaches. Food Chemistry 338:127803.

Sghaier-Hammami B, Hammami S, Baazaoui N, Gómez-Díaz C, Jorrín-Novo JV. 2020. Dissecting the seed maturation and germination processes in the non-orthodox Quercus ilex species based on protein signatures as revealed by 2-DE coupled to MALDI-TOF/TOF proteomics strategy. International Journal of Molecular Sciences 21:4870.

San-Eufrasio B, Sánchez-Lucas R, López-Hidalgo C, Guerrero-Sánchez VM, Castillejo-Sánchez MA, Maldonado-Alconada AM, Jorrín-Novo JV, Rey MD. 2020. Responses and differences in tolerance to water shortage under climatic dryness conditions in seedlings from Quercus spp. and Andalusian Q. ilex populations. Forests 11:707.

Pedrosa, M, Guerrero-Sánchez VM, Canales‐Bueno N, Loli‐Ausejo D, Castillejo-Sánchez MA, Quirce S, Jorrín-Novo JV, Rodríguez‐Perez, R. 2020. Quercus ilex pollen allergen, Que i 1, responsible for pollen food allergy syndrome caused by fruits in Spanish allergic patients. Clinical & Experimental Allergy.

Draeger T, Martín AC, Kader Alabdullah A, Pendle A, Rey MD, Shaw P, Moore G. 2020. Dmc1 is a candidate for temperature tolerance during wheat meiosis. Theoretical and Applied Genetics 133:809-828.

Castillejo-Sánchez MA, Fondevilla-Aparicio S, Fuentes-Almagro C, Rubiales D. 2020. Quantitative Analysis of Target Peptides Related to Resistance Against Ascochyta Blight (Peyronellaea pinodes) in Pea. Journal of Proteome Research 19:1000-1012.

Almeida T, Pinto G, Correia B, Gonçalves S, Meijón M, Escandón M. 2020. In-depth analysis of the Quercus suber metabolome under drought stress and recovery reveals potential key metabolic players. Plant Science 299: 110606.

Amaral J, Correia B, Escandón M, Jesus C, Serôdio J, Valledor-González L, Hancock RD, Dinis L-T, Gomez-Cadenas A, Alves A, Pinto G. 2020. Temporal physiological response of pine to Fusarium circinatum infection is dependent on host susceptibility level: the role of ABA catabolism. Tree Physiology, tpaa143.

Lamelas L, Valledor-González L, Escandón M, Pinto G, Cañal MJ, Meijón M. 2020. Integrative analysis of the nuclear proteome in Pinus radiata reveals thermopriming coupled to epigenetic regulation. Journal of Experimental Botany 71: 2040–2057.

Guerrero-Sánchez VM, Maldonado-Alconada AM, Amil-Ruiz F, Verardi A, Jorrín-Novo JV, Rey MD. 2019. Ion Torrent and lllumina, two complementary RNA-seq platforms for constructing the holm oak (Quercus ilex) transcriptome. PLoS ONE 14:e0210356.

Salas-Moreno M, Contreras-Puentes N, Rodríguez-Cavallo E, Jorrín-Novo JV, Marrugo-Negrete J, Méndez-Cuadro D. 2019. Protein carbonylation as a biomarker of heavy Metal, Cd and Pb, damage in Paspalum fasciculatum Willd. ex Flüggé. Plants, 8:513.

Félix C, Meneses R, Gonçalves MF, Tilleman L, Duarte AS, Jorrín-Novo JV, de Peer YV, Deforce D, Niewwerburhg EF, Esteves AC, Alves, A. 2019. A multi-omics analysis of the grapevine pathogen Lasiodiplodia theobromae reveals that temperature affects the expression of virulence-and pathogenicity-related genes. Scientific Reports, 9:1-12.

Santos C, Nogueira F, Domont GB, Fontes W, Prado GS, Habibi P, Santos VO, Oliveria-Neto OB, Grossi-De-Sá MF, Jorrín-Novo JV, Franco OL, Mehta A. 2019. Proteomic analysis and functional validation of a Brassica oleracea endochitinase involved in resistance to Xanthomonas campestris. Frontiers in Plant Science 10:414.

Romero-Rodríguez MC, Jorrín-Novo JV, Castillejo-Sánchez MA. 2019. Toward characterizing germination and early growth in the non-orthodox forest tree species Quercus ilex through complementary gel and gel-free proteomic analysis of embryo and seedlings. Journal of Proteomics 197:60-70.

Brito C, Dinis L-T, Luzio A, Silva E, Gonçalves A, Meijón M, Escandón M, Arrobas M, Rodrigues MÂ, Moutinho-Pereira J, Correia CM. 2019. Kaolin and salicylic acid alleviate summer stress in rainfed olive orchards by modulation of distinct physiological and biochemical responses. Scientia Horticulturae 246: 201-211.

Romero-Rodríguez MC, Archidona-Yuste A, Abril N, Gil-Serrano AM, Meijón M, Jorrín-Novo JV. 2018. Germination and early seedling development in Quercus ilex recalcitrant and non-dormant seeds: Targeted transcriptional, hormonal, and sugar analysis. Frontiers in Plant Sciences 9:1508.

López-Hidalgo C, Guerrero-Sánchez VM, Gómez-Gálvez IM, Sánchez-Lucas R, Castillejo-Sánchez MA, Maldonado-Alconada AM, Valledor-González L, Jorrín-Novo JV. 2018. A multi-omics analysis pipeline for the metabolic pathway reconstruction in the orphan species Quercus ilex. Frontiers in Plant Sciences 9:935.

Simova-Stoilova LP, López-Hidalgo C, Sánchez-Lucas R, Valero-Galván J, Romero-Rodríguez MC, Jorrín-Novo JV. 2018. Holm oak proteomic response to water limitation at seedling establishment stage reveals specific changes in different plant parts as well as interaction between roots and cotyledons. Plant Science 276:1-13.

Fernández i Martí A, Romero-Rodríguez MC, Navarro-Cerrillo RM, Abril N, Jorrín-Novo JV. 2018. Population genetic diversity of Quercus ilex subsp. ballota (Desf.) Samp. reveals divergence in recent and evolutionary migration rates in the Spanish Dehesas. Forests 9:337

Félix C, Salvatore MM, DellaGreca M, Meneses R, Duarte AS, Salvatore F, Naviglio D, Gallo M, Jorrín-Novo JV, Alves A, Adolfi A, Esteves AC. 2018. Production of toxic metabolites by two strains of Lasiodiplodia theobromae, isolated from a coconut tree and a human patient. Mycologia 110:642-653

Loewe V, Navarro-Cerrillo RM, Lucas RS, Gómez FR, Jorrín-Novo JV. 2018. Variability studies of allochthonous stone pine (Pinus pinea L.) plantations in Chile through nut protein profiling. Journal of Proteomics 175:95-104

Pérez-Mora W, Jorrín-Novo JV, Melgarejo LM. 2018. Substantial equivalence analysis in fruits from three Theobroma species through chemical composition and protein profiling. Food Chemistry 240:496-504

Calderón MC, Rey MD, Martín AC, Prieto P (2018) Homoeologous chromosomes from two Hordeum species can recognize and associate during meiosis in wheat in the presence of the Ph1 locus. Fontiers in Plant Science 9:585

Rey MD, Martín AC, Smedley M, Hayta S, Harwood W, Shaw P, Moore G (2018) Magnesium increases homoeologous crossover frequency during meiosis in ZIP4 (Ph1 gene) mutant wheat-wild relative hybrids. Fontiers in Plant Science 9:509

Rey MD, Moore G, Martín AC (2018) Identification and comparison of individual chromosomes of three accessions of Hordeum chilense, Hordeum vulgare, and Triticum aestivum by FISH. Genome 61:387-396

Watson A, Ghosh S, Williams MJ, Cuddy WS, Simmonds J, Rey MD, et al. (2018) Speed breeding is a powerful tool to accelerate crop research and breeding. Nature Plants 4:23-29

Escandón M, Meijón M, Valledor-González L, Pascual J, Pinto G, Cañal MJ. 2018. Metabolome Integrated Analysis of High-Temperature Response in Pinus radiata. Frontiers in Plant Science 9: 485

Guerrero-Sánchez VM, Maldonado-Alconada AM, Amil-Ruiz F & Jorrín-Novo JV. 2017. Holm oak (Quercus ilex) Transcriptome. De novo Sequencing and Assembly Analysis. Frontiers in Molecular Biosciences 4:70

Carmo LS, Murad AM, Resende RO, Boiteux LS, Ribeiro SG, Jorrín-Novo JV, Mehta A. 2017. Plant responses to tomato chlorotic mottle virus: Proteomic view of the resistance mechanisms to a bipartite begomovirus in tomato. Journal of Proteomics 151:284-292

Rey MD, Martín AC, Higgins J, Swarbreck D, Uauy C, Shaw P, Moore G. (2017) Exploiting the ZIP4 homologue within the wheat Ph1 locus has identified two lines exhibiting homoeologous crossover in wheat-wild relative hybrids. Molecular Breeding 37:95

Rey MD, Prieto P (2017) Detection of alien genetic introgressions in bread wheat using dot-blot genomic hybridisation. Molecular Breeding 37:32

Martín AC, Rey MD, Shaw P, Moore G *Ambos autores han contribuido de la misma manera. (2017) Dual effect of the wheat Ph1 locus on chromosome synapsis and crossover. Chromosoma 126:669-680

Escandón M, Valledor-González L, Pascual J, Pinto G, Cañal MJ, Meijón M. 2017. System-wide analysis of short-term response to high temperature in Pinus radiata. Journal of Experimental Botany 68: 3629–3641

Sghaier-Hammami B, Redondo-López I, Valero-Galván J, Jorrín-Novo JV. 2016. Protein profile of cotyledon, tegument, and embryonic axis of mature acorns from a non-orthodox plant species: Quercus ilex. Planta 243:369-396

Rodríguez de Francisco LE, Romero-Rodríguez MC, Navarro-Cerrillo RM, Minino V, Perdomo O, Jorrín-Novo JV. 2016. Characterization of the orthodox Pinus occidentalis seed and pollen proteomes by using complementary gel-based and gel-free approaches. Journal of Proteomics 143:382-389

Mercado-Blanco J, Alós E, Rey MD, Prieto P (2016) Pseudomonas fluorescens PICF7 displays an endophytic lifestyle in cultivated cereals and enhances yield in barley. FEMS microbiology ecology 126l:669-680

Castillejo-Sánchez MA, Iglesias-García R, Wienkoop S, Rubiales D. 2016. Label-free quantitative proteomic analysis of tolerance to drought in Pisum sativum. Proteomics 16:2776-2787

Lyon D, Castillejo MA, Mehmeti-Tershani V, Staudinger C, Kleemaier C, Wienkoop S. 2016. Drought and Recovery: Independently Regulated Processes Highlighting the Importance of Protein Turnover Dynamics and Translational Regulation in Medicago truncatula. Molecular & Cellular Proteomics 15:10.1074/mcp.M115.049205, 1921–1937

Romero-Rodríguez MC, Abril N, Sánchez-Lucas R, Jorrín-Novo JV. 2015. Multiplex staining of 2-DE gels for an initial phosphoproteome analysis of germinating seeds and early grown seedlings from a non-orthodox spp.: Quercus ilex L. subsp. ballota [Desf.] Samp. Frontiers in Plant Science 6:620

Simova-Stoilova LP, Romero-Rodríguez MC, Sánchez-Lucas R, Navarro-Cerrillo RM, Medina-Auñón A, Jorrín-Novo JV. 2015. 2-DE proteomics analysis of drought treated seedlings of Quercus ilex supports a root active strategy for metabolic adaptation in response to water shortage. Frontiers in Plant Science 6:627

Gelis S, González-Fernández R, Herrera R, Jorrín-Novo JV, Ramos J. 2015. A physiological, biochemical and proteomic characterization of Saccharomyces cerevisiae trk1, 2 transport mutants grown under limiting potassium conditions. Microbiology 161:1260-1270

Rey MD, Calderón MC, Rodrigo MJ, Zacarías L, Alós E, Prieto P (2015) Novel bread wheat lines enriched in carotenoids carrying Hordeum chilense chromosome arms in the ph1b background. PLoS ONE 10:e0134598

Knight E, Binnie A, Draeger T, Moscou M, Rey MD, Sucher J, Mehra S, King I, Moore G (2015) Mapping the ‘breaker’ element of the gametocidal locus proximal to a block of sub-telomeric heterochromatin on the long arm of chromosome 4Ssh of Aegilops sharonensis. Theoretical and Applied Genetics 128:1049

Rey MD, Calderón MC, Prieto P (2015) The use of the ph1b mutant to induce recombination between the chromosomes of wheat and barley. Frontiers in Plant Science 6:160

Castillejo-Sánchez MA, Bani M, Rubiales D. 2015. Understanding pea resistance mechanisms in response to Fusarium oxysporum through proteomic analysis. Phytochemistry 115:44-58

Chaturvedi P, Doerfler H, Jegadeesan S, Ghatak A, Pressman E, Castillejo-Sánchez MA, Wienkoop S, Egelhofer V, Firon N, Weckwerth W. 2015. Heat-Treatment-Responsive Proteins in Different Developmental Stages of Tomato Pollen Detected by Targeted Mass Accuracy Precursor Alignment (tMAPA). Journal of Proteome Research 14:4463-4471

Muhammad, N., Castillejo, M.A., Rey, M.-D. & Jorrín-Novo, J.V. (2023). An overview of oak species in Pakistan: past, present, and future research perspectives. Forests, 14, 777.

Castillejo, M.A., Pascual, J., Jorrín-Novo, J.V. & Balbuena, T.S. (2023) Proteomics research in forest trees: A 2012-2022 update. Frontiers in Plant Sciences, 14, 1130665.

Cortés, A., Castillejo, M.A., Yockteng, R. (2023) ‘Omics’ Approaches for Crop Improvement. Agronomy 13, 1401.

Balbuena, T.S., Castillejo, M.A., Pascual, J. (2023) Forest Tree Proteomics. Frontiers in Plant Science. Aceptado.

Maldonado-Alconada, A. M., Castillejo, M. Á., Rey, M. D., Labella-Ortega, M., Tienda-Parrilla, M., Hernández-Lao, T., … & Jorrin-Novo, J. V. (2022). Multiomics Molecular Research into the Recalcitrant and Orphan Quercus ilex Tree Species: Why, What for, and How. International Journal of Molecular Sciences, 23, 9980.

Jorrín-Novo JV (2021) Proteomics and plant biology: contributions to date and a look towards the next decade, Expert Review of Proteomics. DOI:10.1080/14789450.2021.1910028

Escandón M, Castillejo-Sánchez MA, Jorrín-Novo JV, Rey MD. 2021. Molecular research on stress responses in Quercus spp.: From classical biochemistry to systems biology through omics analysis. Forests 12:364

Rey MD, Castillejo-Sánchez MA, Sánchez-Lucas R, Guerrero-Sánchez VM, López-Hidalgo C, Romero-Rodríguez MC, Valero-Galván J, Sghaier-Hammami B, Simova-Stoilova L, Echevarría-Zomeño S, Jorge I, Gómez-Gálvez IMM, Papa MA, Carvalho K, Rodríguez de Francisco LE, Maldonado-Alconada AM, Valledor-González L, Jorrín-Novo JV. 2019. Proteomics, Holm Oak (Quercus ilex L.) and other recalcitrant and orphan forest tree species: How do they see each other?. International Journal of Molecular Sciences 20:692

Jorrín-Novo JV, Komatsu S, Sánchez-Lucas R, Rodríguez de Francisco LE. 2019. Gel electrophoresis-based plant proteomics: Past, present, and future. Happy 10th anniversary Journal of Proteomics!. Journal of Proteomics 198:1-10

Cabello-Hurtado F, Keller J, Ley J, Sánchez-Lucas R, Jorrín-Novo JV, Aïnouche A. 2016. Proteomics for exploiting diversity of lupin seed storage proteins and their use as nutraceuticals for health and welfare. Journal of Proteomics 143:57-68

Jorrín-Novo JV. 2016. Mike Dunn: Proteomics in Spain, and the field of plant proteomics. Proteomics 16:2842-2844

González-Fernández R, Valero-Galván J, Gómez-Gálvez FJ, Jorrín-Novo JV. 2015. Unraveling the in vitro secretome of the phytopathogen Botrytis cinerea to understand the interaction with its hosts. Frontiers in Plant Science, 6:839

Jorrín-Novo JV. 2015. Scientific standards and MIAPEs in plant proteomics research and publications. Frontiers in Plant Science 6:473

Rubiales D, Fondevilla-Aparicio S, Chen W, Gentzbittel L, Higgins TJV, Castillejo-Sánchez MA, Singh KB, Rispail N. 2015. Achievements and Challenges in Legume Breeding for Pest and Disease Resistance. Critical Reviews in Plant Sciences 34:195-356

Setsuko K, Jorrín-Novo JV. 2021. Plant Proteomic Research 3.0. International Journal of Molecular Sciences. MDPI St. Alban-Anlage 66 4052 Basel, Switzerland.

Jorrín-Novo JV, Valledor-González L, Castillejo-Sánchez MA, Rey MD. 2020. Plant Proteomics: Methods and Protocols. Humana, New York, NY. 3rd Edition

Sghaier-Hammami B, Baazaoui N, Drira R, Drira N, Jorrín-Novo JV (2021). Proteomic Insights of Date Palm Embryogenesis and Responses to Environmental Stress. In: Al-Khayri J.M., Jain S.M., Johnson D.V. (eds) The Date Palm Genome, Vol. 2. Compendium of Plant Genomes. Springer, Cham. Pp: 85-99