Publications

• Tejedor, J. R., Peñarroya, A., Gancedo-Verdejo, J., Santamarina-Ojeda, P., Pérez, R. F., López-Tamargo, S., Díez-Borge A, Alba-Linares J. J., González-Del-Rey N., Urdinguio R. G., Mangas C., Roberti A., López V., Morales-Ruiz T., Ariza R. R., Roldán-Arjona T., Meijón M., Valledor L., Cañal M. J., Fernández-Martínez D., Fernández-Hevia M., Jiménez-Fonseca P., García-Flórez L. J., Fernández A. F., Fraga M. F. (2023). CRISPR/dCAS9-mediated DNA demethylation screen identifies functional epigenetic determinants of colorectal cancer. Clinical Epigenetics, 15(1), 1-17.
• Parrilla-Doblas, J. T., Morales-Ruiz, T., Ariza, R. R., Martínez-Macías, M. I., & Roldán-Arjona, T. (2022). The C-terminal domain of Arabidopsis ROS1 DNA demethylase interacts with histone H3 and is required for DNA binding and catalytic activity. DNA repair, 115, 103341.
• García-Ortiz, M. V., Torre-Aguilar, M. J. D. L., Morales-Ruiz, T., Gómez-Fernández, A., Flores-Rojas, K., Gil-Campos, M., Martín-Borreguero, P., Ariza, R. R., Roldán-Arjona, T., Perez-Navero, J. L. (2021). Analysis of global and local DNA methylation patterns in blood samples of patients with autism spectrum disorder. Frontiers in Pediatrics, 9, 685310.
• Jordano-Raya M, Beltrán-Melero C, Moreno-Recio MD, Martínez-Macías MI, Ariza RR, Roldán-Arjona T, Córdoba-Cañero D. (2021). Complementary Functions of Plant AP Endonucleases and AP Lyases during DNA Repair of Abasic Sites Arising from C:G Base Pairs.International Journal of Molecular Sciences, 22(16):8763.
• Devesa-Guerra, I., Morales-Ruiz, T., Pérez-Roldán, J., Parrilla-Doblas, J. T., Dorado-León, M., García-Ortiz, M. V., Ariza, R. R. and Roldán-Arjona, T. (2020). DNA methylation editing by CRISPR-guided excision of 5-methylcytosine. Journal of molecular biology, 432(7), 2204-2216.
• Córdoba-Cañero, D., Ariza, R. R., and Roldán-Arjona, T. (2020). Base Excision Repair in Plants: Variations on a Theme. In DNA Damage, DNA Repair and Disease (pp. 48-74).
• Parrilla-Doblas, J. T., Roldán-Arjona, T., Ariza, R. R., and Córdoba-Cañero, D. (2019). Active DNA demethylation in plants. International journal of molecular sciences, 20(19), 4683.
• Roldán-Arjona, T., Ariza, R. R., and Córdoba-Cañero, D. (2019). DNA base excision repair in plants: an unfolding story with familiar and novel characters. Frontiers in plant science, 10, 1055.
• Barbado, C., Córdoba-Cañero, D., Ariza, R. R. and Roldán-Arjona, T. (2018). Nonenzymatic release of N7-methylguanine channels repair of abasic sites into an AP endonuclease-independent pathway in Arabidopsis. PNAS. 115(5):E916-E924.
• Morales-Ruiz, T., García-Ortiz, M. V., Devesa-Guerra, I., Raya-Ruiz, L., Tejedor J.R., Bayón G.F., Sierra, M. I., Fraga, M., Ariza, R. R., Roldán-Arjona, T. (2018). DNA methylation reprogramming of human cancer cells by expression of a plant 5-methylcytosine DNA glycosylase. Epigenetics, 13:1-23.
• Córdoba-Cañero, D., Cognat, V., Ariza, R. R., Roldán-Arjona, T. and Molinier, J. (2017). Dual control of ROS1-mediated active DNA demethylation by the DNA DAMAGE BINDING protein 2 (DDB2). Plant J, 92(6):1170-1181.
• Parrilla-Doblas, J.T., Ariza, R.R. and Roldán-Arjona, T. (2017). Targeted DNA demethylation in human cells by fusion of a plant 5-methylcytosine DNA glycosylase to a sequence-specific DNA. Epigenetics, 12(4):296-303.
• Li, Y., Córdoba-Cañero, D., Qian, W., Zhu, X., Tang, K., Zhang, H., Ariza, R.R., Roldán-Arjona, T. and Zhu, J.K. (2015). An AP Endonuclease Functions in Active DNA Demethylation and Gene Imprinting in Arabidopsis. PLoS Genet 11:e1004905. 
• Córdoba-Cañero, D., Roldán-Arjona, T. and Ariza, R. R. (2014). Arabidopsis ZDP DNA 3′‐phosphatase and ARP endonuclease function in 8‐oxoG repair initiated by FPG and OGG1 DNA glycosylases. Plant J, 79:824-834. 
• Ramiro-Merina, A., Ariza R. R., Roldán-Arjona, T. (2013). Molecular characterization of a putative plant homolog of MBD4 DNA glycosylase. DNA Repair, 12(11):890-898. 
• Parrilla-Doblas, J. T., Ponferrada-Marín, M. I., Roldán-Arjona, T., and Ariza, R. R. (2013). Early steps of active DNA demethylation initiated by ROS1 glycosylase require three putative helix-invading residues. Nucleic Acids Res 41(18):8654-8664.
• Martín, M.J., García-Ortiz, M.V., Esteban, V., and Blanco, L. (2013). Ribonucleotides and manganese ions improve non-homologous end joining by human Pol mu. Nucleic Acids Res 41, 2428-2436. 
• Martín, M.J., García-Ortiz, M.V., Gómez-Bedoya, A., Esteban, V., Guerra, S. and Blanco, L. (2013). A specific N-terminal extension of the 8 kDa domain is required for DNA end-bridging by human Pol mu and Pol Lambda. Nucleic Acids Res 41, 9105-9116. 
• Martínez-Macías, M.I., Córdoba-Cañero, D., Ariza, R.R., and Roldán-Arjona, T. (2013). The DNA repair protein XRCC1 functions in the plant DNA demethylation pathway by stimulating 5-meC excision, gap tailoring and DNA ligation. J Biol Chem, 288 (8):5496-5505. 
• Córdoba-Cañero, D., Roldán-Arjona, T., and Ariza, R.R. (2012). Using Arabidopsis cell extracts to monitor repair of DNA base damage in vitro. Methods Mol Biol 920, 263-277.  
• Martínez-Macías, M.I., Qian, W., Miki, D., Pontes, O., Liu, Y., Tang, K., Liu, R., Morales-Ruiz, T., Ariza, R.R., Roldán-Arjona, T., et al. (2012). A DNA 3' phosphatase functions in active DNA demethylation in Arabidopsis. Mol Cell 45, 357-370. 
• Ponferrada-Marín, M.I., Roldán-Arjona, T., and Ariza, R.R. (2012). Demethylation initiated by ROS1 glycosylase involves random sliding along DNA. Nucleic Acids Res 40, 11554-11562. 
• Córdoba-Cañero, D., Roldán-Arjona, T., and Ariza, R.R. (2011). Arabidopsis ARP endonuclease functions in a branched base excision DNA repair pathway completed by LIG1. Plant J 68, 693-702.
• Dubois, E., Córdoba-Cañero, D., Massot, S., Siaud, N., Gakiere, B., Domenichini, S., Guerard, F., Roldán-Arjona, T., and Doutriaux, M.P. (2011). Homologous recombination is stimulated by a decrease in dUTPase in Arabidopsis. PLoS One 6, e18658. 
• García-Ortiz, M.V., Marsin, S., Arana, M.E., Gasparutto, D., Guerois, R., Kunkel, T.A., and Radicella, J.P. (2011). Unexpected role for Helicobacter pylori DNA polymerase I as a source of genetic variability. PLoS Genet 7, e1002152. 
• Ponferrada-Marín, M.I., Parrilla-Doblas, J.T., Roldán-Arjona, T., and Ariza, R.R. (2011). A discontinuous DNA glycosylase domain in a family of enzymes that excise 5-methylcytosine. Nucleic Acids Res 39, 1473-1484. 
• Córdoba-Cañero, D., Dubois, E., Ariza, R.R., Doutriaux, M.P., and Roldán-Arjona, T. (2010). Arabidopsis uracil DNA glycosylase (UNG) is required for base excision repair of uracil and increases plant sensitivity to 5-fluorouracil. J Biol Chem, 285, 7475-7483. 
• Ponferrada-Marín, M.I., Martínez-Macías, M.I., Morales-Ruiz, T., Roldán-Arjona, T., and Ariza, R.R. (2010). Methylation-independent DNA binding modulates specificity of repressor of silencing 1 (ROS1) and facilitates demethylation in long substrates. J Biol Chem, 285, 23032-23039. 
• Ariza, R.R., Roldán-Arjona, M.T., García-Ortiz, M.V., Morales-Ruiz, T., Córdoba-Cañero, D., Ponferrada-Marín, M.I., Martínez-Macías, M.I., Ramiro, A., and Hermoso-Rodríguez, R. (2009a). El papel de la escisión de bases en el mantenimiento del genoma y el epigenoma. In IV Jornadas de Divulgación de la Investigación en Biología Molecular, Celular, Genética y Biotecnología (Universidad de Córdoba), 169-173. 
• Ariza, R.R., Roldán-Arjona, T., García-Ortiz, M.V., Morales-Ruiz, T., Ortega-Galisteo, A.P., Martínez-Macías, M.I., Ponferrada-Marín, M.I., and Schiliro, E. (2009b). Capítulo 6. Desmetilación activa del ADN: un mecanismo epigenético para la reactivación de genes silenciados. In Biotecnología III Jornadas de Divulgación de la Investigación en Biología Molecular, Celular y Biotecnología, G. Dorado Pérez, J.V. Jorrín Novo, M. Tena Aldave, and E. Fernández Reyes, eds. (Córdoba, Universidad de Córdoba), 23-26. 
• Córdoba-Cañero, D., Morales-Ruiz, T., Roldán-Arjona, T., and Ariza, R.R. (2009). Single-nucleotide and long-patch base excision repair of DNA damage in plants. Plant J 60, 716-728. 
• González Roncero, M.I., De la Hera, C., Di Pietro, A., Ruiz Roldán, M.C., N., R., Martínez Rocha, A.L., Córdoba Cañero, D., Martín Urdiroz, M., Prados Rosales, R., Martínez Aguilera, E., et al. (2009). Análisis molecular de la patogénesis en Fusarium oxysporum. In Biotecnología, (Universidad de Córdoba), ed., 36-38. 
• Ponferrada-Marin, M.I., Roldán-Arjona, T., and Ariza, R.R. (2009). ROS1 5-methylcytosine DNA glycosylase is a slow-turnover catalyst that initiates DNA demethylation in a distributive fashion. Nucleic Acids Res 37, 4264-4274. 
• Roldán-Arjona, T., and Ariza, R.R. (2009a). Repair and tolerance of oxidative DNA damage in plants. Mutat Res, 681, 169-179. 
• Roldán-Arjona, T., and Ariza, R.R. (2009b). DNA Demethylation. In In: DNA and RNA Modification Enzymes: Structure, Mechanisms, Functions and Evolution, L. Biosciences, ed., pp. 149-161. 
• Berdasco, M., Alcazar, R., Garcia-Ortiz, M.V., Ballestar, E., Fernandez, A.F., Roldán-Arjona, T., Tiburcio, A.F., Altabella, T., Buisine, N., Quesneville, H., et al. (2008). Promoter DNA hypermethylation and gene repression in undifferentiated Arabidopsis cells. PLoS ONE 3, e3306. 
• Calero, F., Córdoba-Cañero, D., Di Pietro, A., and Gónzalez Roncero, M.I. (2008). Aplicaciones de la proteína verde fluorescente (GFP) para el estudio de los hongos fitopatógenos. In Herramientas Biotecnológicas en Fitopatología, M. Prensa, ed., 285-292. 
• Córdoba-Cañero, D., and Roncero, M.I. (2008a). Functional analyses of laccase genes from Fusarium oxysporum. Phytopathology 98, 509-518. 
• Córdoba-Cañero, D., and Roncero, M.I. (2008b). Influence of the chloride channel of Fusarium oxysporum on extracellular laccase activity and virulence on tomato plants. Microbiology 154, 1474-1481. 
• González Roncero, M.I., Calero, F., Córdoba Cañero, D., and Di Pietro, A. (2008). Transformación genética aplicada al estudio de la patogénesis fúngica. In Herramientas Biotecnológicas en fitopatología. (Mundi Prensa), 227-240. 
• Ortega-Galisteo, A.P., Morales-Ruiz, T., Ariza, R.R., and Roldán-Arjona, T. (2008). Arabidopsis DEMETER-LIKE proteins DML2 and DML3 are required for appropriate distribution of DNA methylation marks. Plant Mol Biol 67, 671-681. 
• Garcia-Ortiz, M.V., Roldán-Arjona, T., and Ariza, R.R. (2007). The noncatalytic C-terminus of AtPOLK Y-family DNA polymerase affects synthesis fidelity, mismatch extension and translesion replication. Febs J 274, 3340-3350. 
• Morales-Ruiz, T., Ortega-Galisteo, A.P., Ponferrada-Marín, M.I., Martínez-Macías, M.I., Ariza, R.R., and Roldán-Arjona, T. (2006). DEMETER and REPRESSOR OF SILENCING 1 encode 5-methylcytosine DNA glycosylases. Proc Natl Acad Sci 103, 6853-6858. 
• García-Ortiz, M.V., Ariza, R.R., Hoffman, P.D., Hays, J.B., and Roldán-Arjona, T. (2004). Arabidopsis thaliana AtPOLK encodes a DinB-like DNA polymerase that extends mispaired primer termini and is highly expressed in a variety of tissues. Plant J 39, 84-97. 
• Morales-Ruiz, T., Binrincioglu, M., Jaruga, P., Rodriguez H., Roldan-Arjona, T., and Dizdaroglu, M. (2003). Arabidopsis thaliana Ogg1 Protein Excises 8-Hydroxyguanine and 2,6-Diamino-4-hydroxy-5-formamidopyrimidine from Oxidatively Damaged DNA Containing Multiple Lesions. Biochemistry 42, 3089-3095. 
• Alejandre-Durán, E., Roldán-Arjona, T., Ariza, R.R., and Ruiz-Rubio, M. (2003). The photolyase gene from the plant pathogen Fusarium oxysporum f. sp. lycopersici is induced by visible light and alpha-tomatine from tomato plant. Fungal Genet Biol 40, 159-165. 
• Roncero, M.I.G., Hera, C., Ruiz-Rubio, M., García Maceira, F.I., Madrid, M.P., Caracuel, Z., Calero, F., Delgado-Jarana, J., Roldán-Rodriguez, R., and Martínez-Rocha, A.L. (2003). Fusarium as a model for studying virulence in soilborne plant pathogens. Physiol Mol Plant P 62, 87-98. 
• Gong, Z., Morales-Ruiz, T., Ariza, R.R., Roldán-Arjona, T., David, L., and Zhu, J.K. (2002). ROS1, a repressor of transcriptional gene silencing in Arabidopsis, encodes a DNA glycosylase/lyase. Cell 111, 803-814. 
• Roldán-Arjona, T., García-Ortiz, M.V., and Ariza, R.R. (2002). DNA repair of oxidative base damage induced by ionising radiation and ultraviolet light. In Recent Research Developments in Photochemistry and Photobiology, S.G. Pandalai, ed. (Trivandrum, Transworld Research Network), 49-62. 
• Ruiz-Rubio, M., Alejandre-Durán, E., and Roldán-Arjona, T., eds. (2002). Recent Research Development in DNA Repair and Mutagenesis. (Trivandrum, India, Research Signpost).
• Shahein, Y.E., Garrido, J.J., Yerle, M., Roldán-Arjona, M.T., Perez de la Lastra, J.M., and de Andrés-Cara, D.F. (2002). Assignment of the CD47 gene to pig chromosome band 13q42-->1/2q46 with somatic cell hybrids. Cytogenet Genome Res 97, 276E.
• García-Ortiz, M.V., Ariza, R.R., and Roldán-Arjona, T. (2001a). An OGG1 orthologue encoding a functional 8-oxoguanine DNA glycosylase/lyase in Arabidopsis thaliana. Plant Mol Biol 47, 795-804. 
• García-Ortiz, M.V., Ariza, R.R., and Roldán-Arjona, T. (2001b). A chemiluminescent method for the detection of DNA glycosylase/lyase activity. Anal Biochem 298, 127-129. ​​
• Perez-Espinosa, A., Roldán-Arjona, T., and Ruiz-Rubio, M. (2001). Pantothenate synthetase from Fusarium oxysporum f. sp. lycopersici is induced by alpha-tomatine. Mol Genet Genomics 265, 922-929. 
• Kuraoka, I., Kobertz, W.R., Ariza, R.R., Biggerstaff, M., Essigmann, J.M., and Wood, R.D. (2000). Repair of an interstrand DNA cross-link initiated by ERCC1-XPF repair/recombination nuclease. J Biol Chem 275, 26632-26636. 
• Wood, R.D., Araujo, S.J., Ariza, R.R., Batty, D.P., Biggerstaff, M., Evans, E., Gaillard, P.H., Gunz, D., Koberle, B., Kuraoka, I., et al. (2000). DNA damage recognition and nucleotide excision repair in mammalian cells. Cold Spring Harb Symp Quant Biol 65, 173-182. 
• Dizdaroglu, M., Karahalil, B., Senturker, S., Buckley, T.J., and Roldán-Arjona, T. (1999). Excision of products of oxidative DNA base damage by human NTH1 protein. Biochemistry 38, 243-246.
• Roldán-Arjona, T., Pérez-Espinosa, A., and Ruiz-Rubio, M. (1999). Tomatinase from Fusarium oxysporum f. sp. lycopersici defines a new class of saponinases. Mol Plant Microbe Interact 12, 852-861. 
• Karahalil, B., Roldán-Arjona, T., and Dizdaroglu, M. (1998). Substrate specificity of Schizosaccharomyces pombe Nth protein for products of oxidative DNA damage. Biochemistry 37, 590-595.
• Melvin, T., Cunniffe, S.M., O'Neill, P., Parker, A.W., and Roldán-Arjona, T. (1998). Guanine is the target for direct ionisation damage in DNA, as detected using excision enzymes. Nucleic Acids Res 26, 4935-4942. 
• Aspinwall, R., Rothwell, D.G., Roldán-Arjona, T., Anselmino, C., Ward, C.J., Cheadle, J.P., Sampson, J.R., Lindahl, T., Harris, P.C., and Hickson, I.D. (1997). Cloning and characterization of a functional human homolog of Escherichia coli endonuclease III. Proc Natl Acad Sci 94, 109-114. 
• Melvin, T., Cunniffe, S., Papworth, D., Roldán-Arjona, T., and O'Neill, P. (1997). Irradiation of DNA with 193 nm light yields formamidopyrimidine-DNA glycosylase(Fpg) protein-sensitive lesions. Photochem Photobiol 65, 660-665. 
• Roldán-Arjona, T., Wei, Y.F., Carter, K.C., Klungland, A., Anselmino, C., Wang, R.P., Augustus, M., and Lindahl, T. (1997). Molecular cloning and functional expression of a human cDNA encoding the antimutator enzyme 8-hydroxyguanine-DNA glycosylase. Proc Natl Acad Sci 94, 8016-8020. 
• Ariza, R.R., Keyse, S.M., Moggs, J.G., and Wood, R.D. (1996). Reversible protein phosphorylation modulates nucleotide excision repair of damaged DNA by human cell extracts. Nucleic Acids Res 24, 433-440. 
• Roldán-Arjona, T., Anselmino, C., and Lindahl, T. (1996). Molecular cloning and functional analysis of a Schizosaccharomyces pombe homologue of Escherichia coli endonuclease III. Nucleic Acids Res 24, 3307-3312. 
• Roldán-Arjona, T., and Sedgwick, B. (1996). DNA base damage induced by ionizing radiation recognized by Escherichia coli UvrABC nuclease but not Nth or Fpg proteins. Mol Carcinog 16, 188-196.
• Sijbers, A.M., de Laat, W.L., Ariza, R.R., Biggerstaff, M., Wei, Y.F., Moggs, J.G., Carter, K.C., Shell, B.K., Evans, E., de Jong, M.C., et al. (1996). Xeroderma pigmentosum group F caused by a defect in a structure-specific DNA repair endonuclease. Cell 86, 811-822. 
• Roldán-Arjona, T., Pueyo, C., and Haynes, R.H. (1995). Mathematical parameters for quantification of mutational responses in bacteria. Mutat Res 346, 77-84. 
• Ariza, R.R., Cohen, S.P., Bachhawat, N., Levy, S.B., and Demple, B. (1994). Repressor mutations in the marRAB operon that activate oxidative stress genes and multiple antibiotic resistance in Escherichia coli. J Bacteriol 176, 143-148. 
• Roldán-Arjona, T., Luque-Romero, F.L., Ariza, R.R., Jurado, J., and Pueyo, C. (1994). Influence of DNA repair by ada and ogt alkyltransferases on the mutational specificity of alkylating agents. Mol Carcinog 9, 200-209. 
• Ruiz-Laguna, J., Ariza, R.R., Prieto-Alamo, M.J., Boiteux, S., and Pueyo, C. (1994). Fpg protein protects Escherichia coli K-12 from mutation induction by the carcinogen 4-nitroquinoline 1-oxide. Carcinogenesis 15, 425-429. ​​
• Ariza, R.R., Roldán-Arjona, T., Hera, C., and Pueyo, C. (1993). A method for selection of forward mutations in supF gene carried by shuttle-vector plasmids. Carcinogenesis 14, 303-305. 
• Roldán-Arjona, T., and Pueyo, C. (1993). Mutagenic and lethal effects of halogenated methanes in the Ara test of Salmonella typhimurium: quantitative relationship with chemical reactivity. Mutagenesis 8, 127-131.
• Abril, N., Roldán-Arjona, T., Prieto-Alamo, M.J., van Zeeland, A.A., and Pueyo, C. (1992). Mutagenesis and DNA repair for alkylation damages in Escherichia coli K-12. Environ Mol Mutagen 19, 288-296.
• Ariza, R.R., Serrano, A., and Pueyo, C. (1992a). Direct-acting mutagenic activity in white, rose, and red wines with the Ara test of Salmonella typhimurium. Environ Mol Mutagen19, 14-20. 
• Ariza, R.R., Serrano, A., and Pueyo, C. (1992b). Study on the mutagenicity of brandy with the Ara test. Mutagenesis 7, 77-81. 
• Ariza, R.R., and Pueyo, C. (1991). The involvement of reactive oxygen species in the direct-acting mutagenicity of wine. Mutat Res 251, 115-121. 
• Roldán-Arjona, T., Garcia-Pedrajas, M.D., Luque-Romero, F.L., Hera, C., and Pueyo, C. (1991). An association between mutagenicity of the Ara test of Salmonella typhimurium and carcinogenicity in rodents for 16 halogenated aliphatic hydrocarbons. Mutagenesis 6, 199-205.
• Roldán-Arjona, T., and Ariza, R.R. (1990). Ensayos de productos mutagénicos. In Prácticas de Genética, A. Jiménez-Sánchez, ed. (Barcelona, Promociones y Publicaciones Universitarias), 41-44.
• Roldán-Arjona, T., Luque-Romero, F.L., Ruiz-Rubio, M., and Pueyo, C. (1990). Quantitative relationship between mutagenic potency in the Ara test of Salmonella typhimurium and carcinogenic potency in rodents. A study of 11 direct-acting monofunctional alkylating agents. Carcinogenesis 11, 975-980.
• Roldán-Arjona, T., Ruiz-Rubio, M., and Pueyo, C. (1990). Influence of S9 mix on the expression of mutants in the L-arabinose resistance test of Salmonella typhimurium. Mutat Res 243, 303-308. 
• Roldán-Arjona, T., Ruiz-Rubio, M., and Pueyo, C. (1989). Simple method for precise determination of chemical lethality in the L-arabinose resistance test of Salmonella typhimurium. Mutat Res 226, 175-180. 
• Ariza, R.R., Dorado, G., Barbancho, M., and Pueyo, C. (1988). Study of the causes of direct-acting mutagenicity in coffee and tea using the Ara test in Salmonella typhimurium. Mutat Res 201, 89-96. 
• Dorado, G., Ariza, R.R., and Pueyo, C. (1988). Mutagenicity of red wine in the L-arabinose resistance test with Salmonella typhimurium. Mutagenesis 3, 497-502. 
• Roldán-Arjona, T., Ariza, R.R., and Pueyo, C. (1988). Genotoxicidad en sistemas procarióticos. Revista de Toxicología 5, 19-25.